A Play.com survey ahead of Sunday's London Marathon found the Freddie Mercury tune was top of the playlists of those preparing for the 26-mile run, as well as those preferring to do their sprinting on the gym treadmill.
Those responding to the poll also strongly disagreed with New York marathon organisers who last year banned iPods and other digital music players on safety grounds. A whopping 75 per cent of Play.com users said not being able to run to music would affect their performance and lap times.
Robert, from Surrey Quay, said: "I’m training for this years London Marathon and I’ve got one ipod of songs which I’ve just downloaded just to listen to while I’m running"
Also in the survey's top 10 tracks was another Queen favourite, Under Pressure, sung with David Bowie. Plus, the theme from the Rocky movies, Eye of the Tiger by Survivor, as well as Stronger by Kanye West.
But it seems 80s classics are big on the running circuit with Chesney Hawkes' The One and Only, Push It by Salt 'n Pepa and Snap's I've Got The Power among those chosen.
Stranger playlist entries included Tony Christie and Is This The Way To Amarillo, The Proclaimers song I Would Walk 500 Miles and Breaking Free, from Disney's High School Musical.
The full Top 10 was:
1) Don't Stop Me Now (Queen)
2) Born to Run (Bruce Springsteen)
3) Stronger (Kanye West)
4) Start Me Up (Rolling Stones)
5) Theme from Rocky
6) Eye of the Tiger (Survivor)
7) Let's Get It Started (Black Eyed Peas)
8) Under Pressure (Queen/David Bowie)
9) Don’t Cha (Pussy Cat Dolls)
10) Right Here Right Now (Fat Boy Slim)
Helen Marquis, Head of Music for Play.com said: “Music has always been an inspiration for competitors and so it makes sense that we have seen an increase in downloads of songs with a running theme in the last month on PlayDigital”.